E-001 - INVESTIGATION OF THE RELATIONSHIP BETWEEN LEPR GENOTYPE-LEPTIN-GUT MICROBIOTA COMPOSITION IN HEALTHY NON-OBESE KOREAN ADULTS.

J. Kim¹, J. Sunwoo², Y. Cha¹, E. Jin¹, I. Chang¹, J. Jung³, J. Hong¹; ¹Chungnam National University, , , ²Chungnam National University Hostpial, , , ³Chungnam National University Hospital, , .

Background: The LEPR (leptin receptor) genotype has been linked to obesity, and there are discernible variations in the composition of the gut microbiome between individuals who are obese and those who are not. However, the influence of the LEPR genotype on the composition of the gut microbiome in humans remains largely unexplored.

Methods: This research investigated the potential relationship between LEPR single nucleotide polymorphisms (rs1173100, rs1137101, rs790419) and the composition of the gut microbiome in 65 non-obese Korean adults. Comprehensive assessments of various factors, including leptin, triglyceride, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels were also conducted.

Results: The mean±SD (standard deviation) age of the participants was 35.2±8.1 years, body mass index of 21.4±1.8 kg/m2, and leptin level was 7989.1±6687.4 pg/mL. Through 16S rRNA sequencing, 11 phyla were identified. The relative abundance (RA) of Candidatus Saccharibacteria was notably higher in individuals with the G/A genotype compared to those with the G/G genotype for the rs1137101 (P=0.0322). Body mass index and leptin levels did not exhibit substantial differences between individuals with the rs1137101 G/G and G/A genotypes. Moreover, there was a significant positive correlation between the RA of Fusobacteria and Tenericutes and leptin level (P=0.0036 and P=0.0000, respectively).

Conclusion: In conclusion, this research suggests a potential connection between LEPR genotype and the composition of the gut microbiome, even among individuals within the normal weight range of the Korean population. This research findings were published online in Biomolecules & Therapeutics on July 28, 2023. (https://doi.org/10.4062/biomolther.2023.116)